Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Journal of Applied Polymer Science, 72, 871-876. dextran hydrodynamic radius Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte The .gov means its official. 2. 2019;26(1):25261. and transmitted securely. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Careers. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Regulating dynamic signaling between hematopoietic stem cells Journal of Physical Chemistry. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Polysaccharide hydrogels for modified release formulations. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Thermo Fisher Scientific. Treatment of acne scars by fractional bipolar radiofrequency energy. Journal Argentine Chemical Society 100 (2013) 25-34. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). Article 2011;16(2):518. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. Elias, Hans-Georg (1975). Molecular dynamics simulations on aqueous 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. It is well tolerated on parenteral infusion by most people but a very low incidence Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. where tr is the column retention time in minutes (2 of 0.9792). Format), Citation-(BibTeX
ACS Nano. Dextrans can function efficiently as anterograde or retrograde tracers, For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. where (poise) is the viscosity, the (g/cm3) fluid density, and t the liquid draining time (s) subscript zero is referred to solvent, and without subscript is referred to the solution [40], and inherent viscosity is i = r -1. Arond L.H. dextran hydrodynamic radius The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Hydrodynamic Pharm Res. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Determination of intrinsic viscosities of macromolecules and nanoparticles. Citation-(RIS
Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should (1958). Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. 113, 81-86. He, L.; Niemeyer, B. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Alternatives to animal testing: a review. Global hydrodynamic analysis of the molecular flexibility of galactomannans. The solution was measured using an Anton Paar DMA35N densitometer. Levin G, Gershonowitz A, Sacks H, Stern M, Sherman A, Rudaev S, Zivin I, Phillip M. Transdermal delivery of human growth hormone through RF-microchannels. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Indo AM J Pharm. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. Federal government websites often end in .gov or .mil. Journal of American Chemical Society, 64(11), 2716-2718. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. Microporation techniques for enhanced delivery of therapeutic agents. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. California Privacy Statement, WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Intrinsic viscosity molecular weight relationship. Protein Hydrodynamics with Light Scattering Structural and biocompatibility properties of dextran from The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. ; De Jong, S.J. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. Drug Discov Today Technol. Stokes radius This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost Viscosity of Liquids. 2013;3(5):3588605. Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro Wonmi-gu, Bucheon-si, Gyeonggi-do, 14662, Republic of Korea, LG electronics, 19 Yangjae-daero 11-gil, Seocho-gu, Seoul, 06772, Republic of Korea, You can also search for this author in In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. Rheology of dextran solutions. Accessibility Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. 8600 Rockville Pike J. Phys. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. This site needs JavaScript to work properly. For data requests, please contact the authors. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Recent Pat Drug Deliv Formul. After 24h, the cumulative permeation of FITC-dextran M.W. Clipboard, Search History, and several other advanced features are temporarily unavailable. Sachdeva V, Banga AK. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Int J Food Sci Nutr. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Carbohydrate Polymers, 79, 380-390. Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. (2003). TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. The authors declare that they have no competing interests. official website and that any information you provide is encrypted @ Nilimesh See attached. Ficoll and dextran vs. globular proteins as probes for testing Characterisation of insulin analogues therapeutically available to On single point determination of intrinsic viscosity. 2015;23(3):2239. Journal of Physical Chemistry, 58(11), 953-957. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Hydrodynamic Properties of Tragacanthin. Pharm Res. Manage cookies/Do not sell my data we use in the preference centre. Ubbelohde, Leo. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. 7 8 Principles of Size Exclusion Chromatography SEC (GFC The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Anal. De Belder, A. N. (2001). Cookies policy. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Webdextran hydrodynamic radius. European Polymer Journal, 36, 2143-2146. Harding, Stephen E. (1997). Carbohydrate Polymers, 39, 377-380. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Gekko, K. (1971). Amersham Biosciences, AA Edition, www.amershambiosciences.com. J Control Release. volume24, Articlenumber:22 (2020) Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. 2012;101(8):28619. Keywords: At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). European Biophysical Journal, 36, 85-94. J. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Biomater Res 24, 22 (2020). Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. dextran of different origins. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. Front Microbiol. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Study of temperature influence. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Epub 2012 May 28. Bethesda, MD 20894, Web Policies National Library of Medicine Google Scholar. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. eCollection 2015. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. The hydrodynamic radius of 2-NBDG glucose was too small to measure. Table 2 compares the properties of nanoparticles coated with one or other polymer. They concluded that a power law behavior seems to be approached only in the small molecular weight region. CAS Unable to load your collection due to an error, Unable to load your delegates due to an error. Would you like email updates of new search results? Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. We observe that this maximum radius is below Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). In addition, the permeation rate was higher in pig skin than in human skin. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. There is a deviation from linearity after 580kDa [69, 70]. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Synthesis and characterization of blue dextrans. Springer Nature. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Weissella: An Emerging Bacterium with Promising Health Benefits. Durand, A. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. 4D imaging reveals mechanisms of clay-carbon protection and The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. MeSH terms Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Not for use in diagnostic procedures. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Kasaai, Mohammad R. (2007). The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Dilute solution properties and degree of chain branching for dextran. Dextran produced from Weissella cibaria JAG8 was purified and characterized. ; Aberle, T; Burchard, W. (2000). J Cosmet Laser Ther. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. This study was supported by the LG Electronics. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). dextran hydrodynamic radius Progr. Chem. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Figure4 shows the histological analysis of micropores in human cadaver skin. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Bookshelf sharing sensitive information, make sure youre on a federal The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24).